Coding

Part:BBa_K1921001:Design

Designed by: Zhuozhi Chen   Group: iGEM16_TJUSLS_China   (2016-09-24)


MHETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 247
    Illegal AgeI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because we want to express PETase in E.coli and pichia pastor,we make codon optimization on it.



Source

We order the part DNA from a synthesis company.


References